Association study of calpain-10 Gene Poly- morphism in Patients with Polycystic Ovarian Syndrome

Polycystic Ovarian syndrome (PCOS) is a common endocrine disorder with a global prevalence of 5-10% among women of reproductive age. PCOS is characterized by chronic anovulation, polycystic ovaries, hyperandrogenism, hirsutism, overweight, insulin resistance, and infertility. It is well-known that PCOS is a complex trait similar to type-2 diabetes in which both genetic and environmental factors play a crucial role in the pathogenesis of the disease. A couple of type-2 diabetes susceptibility genes including those for insulin secretion and action such as Calpain-10 have shown considerable contribution to genetic predisposition to PCOS. Since Calpain-10 gene seems to be a strong candidate gene for PCOS, we aimed to investigate the role of Caplain-10 gene polymorphism UCSNP-44 (T/C) located in a transcription enhancer element of the gene in disease susceptibility. We carried out a cross-sectional case-control study. Using simple random sampling, ninety healthy women were selected. All ninety patients fulfilled the 2003 Rotterdam criteria of PCOS. The subjects were genotyped for Caplain-10 gene polymorphism UCSNP-44 (T/C) using PCR-RFLP. Differences in genotype distributions between case and control subjects were examined via the chi-square test. Although PCOS showed a high prevalence among women in Bandar Abbas, Caplain-10 gene polymorphism UCSNP-44 (T/C) UCSNP44 did not influence the susceptibility to PCOS.